What's new in USCloning 1.0.50
Dec 8, 2016
- Fixed a bug, introduced in Ver 1.0.49, that causes an error after cutting/copying part of the sequence
New in USCloning 1.0.49 (Nov 28, 2016)
- Import Protein´s files improved.
- Fixed a bug that can crash the program when the "ORF Options menu" is loaded and no molecule was loaded.
- INFO Box width can be changed by the user to be adapted better to the computer screen
New in USCloning 1.0.48 (Mar 14, 2016)
- Fixed a bug that can crash the program when the "Restriction Options menu" (or the Primer Options menu") is changed and no molecule was loaded.
- Fixed a bug, introduced in Ver 1.0.47, affecting the remaping of restriction enzymes or primers/motifs when the loaded molecule was modified.
- Fixed a bug affecting the Restriction Analysis when a new analysis is carried out after changing the Restriction Enzyme Library
New in USCloning 1.0.47 (Apr 23, 2015)
- Allows mapping of Restriction Enzymes and Primers/Motifs showing only those within selected Features or within a selected range of DNA.
New in USCloning 1.0.46 (Apr 23, 2015)
- Fixed a bug affecting the sequence of proteins after running "Best Oligonucleotides from Protein"
New in USCloning 1.0.45 (Feb 5, 2015)
- DNA digestions with enzymes making two double-stranded cuts corrected.
- Restriction analysis affecting enzymes making two double-stranded cuts corrected.
- Some minor bugs fixed.
- Help file updated.
New in USCloning 1.0.44 (Jan 14, 2015)
- Added warning when cutting with an enzyme that makes 2 double-stranded cuts on both sides of the recognized sequence
- Enzyme Site View and Motif Site View windows improved
- Modified some PopUp menus to ease edition
- Undo/Redo allowed when the Restriction Map and/or the Motif Map were changed.
- Fixed a minor bug affecting electrophoresis simulation.
New in USCloning 1.0.43 (Oct 15, 2014)
- Fixed a bug, introduced with Ver 1.0.41, that prevents some ligation reactions
New in USCloning 1.0.42 (Jul 21, 2014)
- Help file updated.
- Menu Operations/Search ORFs: now the search is not updated if the conditions of analysis were not changed.
- Some minor bugs fixed.
New in USCloning 1.0.41 (Apr 24, 2014)
- Chew-back cloning improved (for "Gibson assembly"). The only requirement now is that the protruding ends must have a hybridization stretch with a Tm >= 30ºC. In previous versions, "chew-back cloning" required both, equal size of the protruding ends and complete complementarity.
- Help file updated.
New in USCloning 1.0.40 (Mar 14, 2014)
- View ORF improved
- View circular Map improved
- Recombination window slightly changed
- Added Gateway recombination sites
- Fixed a bug that crashed the program when adding a Feature to a Feature Library marked as Read-Only
- Fixed a bug in Map View occurred when changes were done and Undo/Redo was disabled.
New in USCloning 1.0.39 (Jan 21, 2014)
- Added Menu Libraries to ease the libraries handling
- Fixed a bug that unabled PCR menu when the primers were added manually
- Fixed a bug that was preventing from seeing, in the DNA sequence, any
- restriction enzyme located at base = 1
New in USCloning 1.0.38 (Dec 17, 2013)
- ORFs: No need of rotating circular molecules to detect ORFs passing through the origin.
- Map view: Added a PopUp box that informs on Features/Restriction enzymes/Motifs.
- Map view: Color and map position of features handled individually.
- In previous versions all features had colors and positions of the default type.
- SetUp: Library files for DNA and Proteins are handled independently.
- No need to ask to change the motif library when changing from a protein file to a DNA file or viceversa.
New in USCloning 1.0.37 (Dec 17, 2013)
- Added easy edition of files containing "Recombination Sites" for
- recombinational cloning.
New in USCloning 1.0.36 (Dec 17, 2013)
- Fixed a bug that cracks the program when adding as Feature a searched
- sequence.
- This crack was caused only in rare cases (the found sequence should appear
- more than once, and besides, there should be restriction enzymes or/and
- motifs mapped and there should be changes in the data file that had not been
- saved).
New in USCloning 1.0.35 (Dec 17, 2013)
- Import/Export EMBL/GenBank Files improved.
- Fixed USC Files incompatibility due to Windows Regional Configuration.
- Added alert for Dam/Dcm methylation.
- Some minor bugs fixed.