DeNovoGUI

Proteomics research is a highly complex domain that demands both well-trained professionals and advanced digital tools. The rise in quantitative methods, especially statistical in nature, has led to an increased level of reliance upon programs that analyze peptides.

DeNovoGUI is a software application thatcan help professionals with the analysis of protein sequences, as it is a graphical front-end for de novo sequencing of tandem mass spectra. In this respect, the application supports three sequencing methods: PepNovo+, DirecTag and pNovo+.

For any research to be conducted, it should, however, be noted that the program is resource-intensive and also relies on the latest version of Java. Users who have problems getting the tool to run should install the latest x64 bit version of Java.

Leaving tech specs to the side, the application can process spectrum files in standard MS/MS container MGF formats. Several settings can be adjusted for improved results, including the precursor and mass tolerances and adjustments weights. Fixed and variable modifications can be defined and one can even create new user-defined modifications.

After all the input parameters have been defined, a simple mouse click on the “Start Sequencing!” button allows users to view highly detailed results. These comprise de novo sequence matches, as well as N-Gap and C-Gap values. One of the great features of this program is the ability to generate detailed graphs for each sequence. These charts can be exported to various common formats, including PDFs and PNGs.

To conclude, DeNovoGUI is a good tool for chemists, biologists, physicists, and all other professionals working in the field of Proteomics. It worked without error on the latest Windows version in our testing and had minimal impact on system performance.