QTube

QTube is a Java-based, easy-to-use application that quantitatively assesses the consistency of gating data across a panel of tubes.

QTube produces a numerical as well as a graphical assessment of gate data consistency. The algorithm operates directly on list-mode FCS data and requires no operator intervention.

The program looks at the similarity of event distributions in two or more tubes. Without you needing to direct its attention. QTube computes a fingerprint for each tube, and then compares each fingerprint against the aggregate.

It provides feedback as to how similar the distributions are, which ones (if any) are outliers, and by how much. It does all of this with a single click. If you like, you can easily export the result to Excel or other analysis or reporting software.