X2XML

X2XML is a free and open-source application that enables you to convert files with mass spectrometry data into mzXML file type, as long as you have the appropriate vendor software installed.

The lightweight program represents an extension for the ReAdW tool and offers support for Xcalibur .raw, Agilent .wiff, Micromass .dat (inside .raw folders), Bruker .acqu, Bruker .ascii, PNNL ion-mobility spectrometry (IMS) .imf, and Bruker FTICR S-folders, which are a single-file per spectrum representation of large S files made by Bruker FTICR.

.NET Framework 1.1 must be installed before setting up this software utility. It's wrapped in a simple interface consisting of a standard window that shows all options available.

It's possible to pick the S and IMS folders, select the file with the mass spectrometry data you want to convert, edit the dataset name, and choose the preferred dataset type.

Moreover, you can specify the minimum and maximum values to take into account for the m/z filter range or scan range to exclude very small or very large m/z values and reduce the size of the output file, convert the file with one click, or unzip and convert the item.

Please note that the program is no longer supported and cannot be installed on operating systems newer than Windows Vista. It works only with .NET Framework 1.1 and doesn't support newer .NET Framework versions.